@article{HoferickSch{\"o}nherrBarbe2023,
  author    = {Hoferick, Ratka and Sch{\"o}nherr, Holger and Barbe, St{\´e}phan},
  title     = {Visualization of the Two-Phase Flow Behavior Involved in Enhanced Dense Phase Carbon Dioxide Pasteurization by Means of High-Speed Imaging},
  journal   = {Fluids},
  volume    = {9},
  number    = {1},
  editor    = {Kunugi, Tomoaki},
  issn      = {2311-5521},
  doi       = {10.3390/fluids9010010},
  institution = {Angewandte Naturwissenschaften (F11)},
  pages     = {9},
  year      = {2023},
  abstract  = {This research explores the two-phase flow behavior involved in enhanced dense phase carbon dioxide inactivation of <italic>E. coli</italic> DH5α, which has been shown to possess a high microbial reduction efficiency of up to 3.7 ± 0.4 log. We present an experiment in which the liquid sample was pressurized with liquid carbon dioxide to 8.2 MPa and, after saturation, was forced to flow through a mini tube. An experimental setup was developed to visualize the flow patterns (plug, slug and churn flows) occurring in the mini tube by means of high-speed imaging. The values of the wall shear stress were estimated within the mini tube with the help of the gas slug velocities (8-9 m/s) and were compared with threshold shear stress values reported for the disruption of fresh <italic>E. coli</italic> cells. The results suggest that the preliminary pressurization phase may cause a substantial destabilization of the cell wall of <italic>E. coli</italic> DH5α.},
  language  = {en}
}