TY - JOUR
U1 - Wissenschaftlicher Artikel
A1 - Hoferick, Ratka
A1 - Schönherr, Holger
A1 - Barbe, Stéphan
ED - Kunugi, Tomoaki
T1 - Visualization of the Two-Phase Flow Behavior Involved in Enhanced Dense Phase Carbon Dioxide Pasteurization by Means of High-Speed Imaging
JF - Fluids
N2 - This research explores the two-phase flow behavior involved in enhanced dense phase carbon dioxide inactivation of E. coli DH5α, which has been shown to possess a high microbial reduction efficiency of up to 3.7 ± 0.4 log. We present an experiment in which the liquid sample was pressurized with liquid carbon dioxide to 8.2 MPa and, after saturation, was forced to flow through a mini tube. An experimental setup was developed to visualize the flow patterns (plug, slug and churn flows) occurring in the mini tube by means of high-speed imaging. The values of the wall shear stress were estimated within the mini tube with the help of the gas slug velocities (8–9 m/s) and were compared with threshold shear stress values reported for the disruption of fresh E. coli cells. The results suggest that the preliminary pressurization phase may cause a substantial destabilization of the cell wall of E. coli DH5α.
KW - High-Speed Imaging
KW - Multiphase Flow
KW - Experimental Fluid Mechanics
KW - Dense Phase Carbon Dioxide
KW - Microbial Inactivation
KW - Mechanical Effects
Y1 - 2023
UN - https://nbn-resolving.org/urn:nbn:de:hbz:832-epub4-26604
SN - 2311-5521
SS - 2311-5521
U6 - https://doi.org/10.3390/fluids9010010
DO - https://doi.org/10.3390/fluids9010010
VL - 9
IS - 1
SP - 9
S1 - 9
PB - MDPI
ER -