TY - JOUR U1 - Wissenschaftlicher Artikel A1 - Hoferick, Ratka A1 - Schönherr, Holger A1 - Barbe, Stéphan ED - Kunugi, Tomoaki T1 - Visualization of the Two-Phase Flow Behavior Involved in Enhanced Dense Phase Carbon Dioxide Pasteurization by Means of High-Speed Imaging JF - Fluids N2 - This research explores the two-phase flow behavior involved in enhanced dense phase carbon dioxide inactivation of E. coli DH5α, which has been shown to possess a high microbial reduction efficiency of up to 3.7 ± 0.4 log. We present an experiment in which the liquid sample was pressurized with liquid carbon dioxide to 8.2 MPa and, after saturation, was forced to flow through a mini tube. An experimental setup was developed to visualize the flow patterns (plug, slug and churn flows) occurring in the mini tube by means of high-speed imaging. The values of the wall shear stress were estimated within the mini tube with the help of the gas slug velocities (8–9 m/s) and were compared with threshold shear stress values reported for the disruption of fresh E. coli cells. The results suggest that the preliminary pressurization phase may cause a substantial destabilization of the cell wall of E. coli DH5α. KW - High-Speed Imaging KW - Multiphase Flow KW - Experimental Fluid Mechanics KW - Dense Phase Carbon Dioxide KW - Microbial Inactivation KW - Mechanical Effects Y1 - 2023 UN - https://nbn-resolving.org/urn:nbn:de:hbz:832-epub4-26604 SN - 2311-5521 SS - 2311-5521 U6 - https://doi.org/10.3390/fluids9010010 DO - https://doi.org/10.3390/fluids9010010 VL - 9 IS - 1 SP - 9 S1 - 9 PB - MDPI ER -