@article{CoenenFerrerJaegeretal.2023,
  author    = {Coenen, Anna and Ferrer, Manuel and Jaeger, Karl-Erich and Sch{\"o}rken, Ulrich},
  title     = {Synthesis of 12-aminododecenoic Acid by Coupling Transaminase to Oxylipin Pathway Enzymes},
  journal   = {Applied Microbiology and Biotechnology},
  volume    = {107},
  number    = {7},
  issn      = {0175-7598},
  doi       = {10.1007/s00253-023-12422-6},
  institution = {Angewandte Naturwissenschaften (F11)},
  pages     = {2209 -- 2221},
  year      = {2023},
  abstract  = {Biobased polymers derived from plant oils are sustainable alternatives to petro based polymers. In recent years, multienzyme cascades have been developed for the synthesis of biobased ω-aminocarboxylic acids, which serve as building blocks for polyamides. In this work, we have developed a novel enzyme cascade for the synthesis of 12-aminododeceneoic acid, a precursor for nylon-12, starting from linoleic acid. Seven bacterial ω-transaminases (ω-TAs) were cloned, expressed in Escherichia coli and successfully purified by affinity chromatography. Activity towards the oxylipin pathway intermediates hexanal and 12-oxododecenoic acid in their 9(Z) and 10(E) isoforms was demonstrated for all seven transaminases in a coupled photometric enzyme assay. The highest specific activities were obtained with ω-TA from Aquitalea denitrificans (TRAD), with 0.62 U mg-1 for 12-oxo-9(Z)-dodecenoic acid, 0.52 U mg-1 for 12-oxo-10(E)-dodecenoic acid and 1.17 U mg-1 for hexanal. A one-pot enzyme cascade was established with TRAD and papaya hydroperoxide lyase (HPLCP-N), reaching conversions of 59\% according to LC-ELSD quantification. Starting from linoleic acid, up to 12\% conversion to 12-aminododecenoic acid was achieved with a 3-enzyme cascade comprising soybean lipoxygenase (LOX-1), HPLCP-N and TRAD. Higher product concentrations were achieved by the consecutive addition of enzymes compared to simultaneous addition at the beginning.},
  subject      = {Polyamide},
  language  = {en}
}