TY  - CHAP
U1  - Buchbeitrag
A1  - Kleiner, Beatrice
A1  - Ananaba, Dominik
A1  - Schulz, Sven
A1  - Schörken, Ulrich
T1  - Pseudozyma antarctica lipase B catalysis in deep eutectic solvents
T2  - Book of Proceedings of STEPsCON 2018
N2  - Pseudozyma antarctica Lipase B catalyzed esterification and transesterification in deep eutectic solvents (DES) was investigated in reaction systems with alcohols of different polarity. Coconut oil and crude biodiesel were deacidified successfully with non-immobilized CALBL and final acid values of 1.2 for biodiesel and 0.5 for coconut oil were obtained, while no esterification with ethanol was observed without DES. Water depletion of the lipid phase in the presence of water adsorbing DES causes this difference. Analysis of water contents revealed a 10 fold lower water content of the lipid phase in the presence of a second DES phase than in trials without utilization of DES. In contrast reactions of hydrophilic polyols are suppressed in the presence of DES. While the esterification of fructose and the transesterification with glycerol worked well in the polar solvent 2-methyl-2-butanol, almost no fructose esterification and a decreased transesterification with glycerol were observed in the presence of DES. Analysis of logP values of the substrates explains the substrate dependent differences in reactivity. The polar alcohols are probably bound strongly in the hydrogen-bonding network of the DES phase and are thus not available for lipase catalyzed reactions.
KW  - Lipase
KW  - Deep eutectic solvents
KW  - CALB
KW  - Transesterification
KW  - Esterification
Y1  - 2019
U6  - https://nbn-resolving.org/urn:nbn:de:hbz:832-epub4-14054
UN  - https://nbn-resolving.org/urn:nbn:de:hbz:832-epub4-14054
SP  - S. 18-29
S1  - S. 18-29
ER  -