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Pseudozyma antarctica lipase B catalysis in deep eutectic solvents

  • Pseudozyma antarctica Lipase B catalyzed esterification and transesterification in deep eutectic solvents (DES) was investigated in reaction systems with alcohols of different polarity. Coconut oil and crude biodiesel were deacidified successfully with non-immobilized CALBL and final acid values of 1.2 for biodiesel and 0.5 for coconut oil were obtained, while no esterification with ethanol was observed without DES. Water depletion of the lipid phase in the presence of water adsorbing DES causes this difference. Analysis of water contents revealed a 10 fold lower water content of the lipid phase in the presence of a second DES phase than in trials without utilization of DES. In contrast reactions of hydrophilic polyols are suppressed in the presence of DES. While the esterification of fructose and the transesterification with glycerol worked well in the polar solvent 2-methyl-2-butanol, almost no fructose esterification and a decreased transesterification with glycerol were observed in the presence of DES. Analysis of logP values of the substrates explains the substrate dependent differences in reactivity. The polar alcohols are probably bound strongly in the hydrogen-bonding network of the DES phase and are thus not available for lipase catalyzed reactions.

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Author:Beatrice Kleiner, Dominik Ananaba, Sven Schulz, Ulrich Schörken
Parent Title (English):Book of Proceedings of STEPsCON 2018
Document Type:Part of a Book
Date of first Publication:2019/09/06
Date of Publication (online):2019/09/17
Tag:CALB; Deep eutectic solvents; Esterification; Lipase; Transesterification
Page Number:S. 18-29
Institutes:Angewandte Naturwissenschaften (F11)
CCS-Classification:A. General Literature
Dewey Decimal Classification:500 Naturwissenschaften und Mathematik
JEL-Classification:Z Other Special Topics
Open Access:Open Access
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International