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Transposon Vector-Mediated Stable Gene Transfer for the Accelerated Establishment of Recombinant Mammalian Cell Pools Allowing for High-Yield Production of Biologics

  • Stable recombinant mammalian cells are of growing importance in pharmaceutical biotechnology production scenarios for biologics such as monoclonal antibodies, growth and blood factors, cytokines and subunit vaccines. However, the establishment of recombinant producer cells using classical stable transfection of plasmid DNA is hampered by low stable gene transfer efficiencies. Consequently, subsequent selection of transgenic cells and the screening of clonal cell populations are time- and thus cost-intensive. To overcome these limitations, expression cassettes were embedded into transposon-derived donor vectors. Upon the co-transfection with transposase-encoding constructs, elevated vector copy numbers stably integrated into the genomes of the host cells are readily achieved facilitating under stringent selection pressure the establishment of cell pools characterized by sustained and high-yield recombinant protein production. Here, we discuss some aspects of transposon vector technologies, which render these vectors promising candidates for their further utilization in the production of biologics.
Author:Natalie Tschorn, Karen Berg, Jörn Stitz
Parent Title (English):Biotechnology Letters
Publisher:Springer Netherlands
Document Type:Article
Date of first Publication:2020/07/01
Date of Publication (online):2023/06/01
Tag:Mammalian Cells; Protein Production; Transposon Vector
Page Number:10
Institutes:Angewandte Naturwissenschaften (F11)
Dewey Decimal Classification:500 Naturwissenschaften und Mathematik
Open Access:Open Access
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International