Visualization of the Two-Phase Flow Behavior Involved in Enhanced Dense Phase Carbon Dioxide Pasteurization by Means of High-Speed Imaging
- This research explores the two-phase flow behavior involved in enhanced dense phase carbon dioxide inactivation of <italic>E. coli</italic> DH5α, which has been shown to possess a high microbial reduction efficiency of up to 3.7 ± 0.4 log. We present an experiment in which the liquid sample was pressurized with liquid carbon dioxide to 8.2 MPa and, after saturation, was forced to flow through a mini tube. An experimental setup was developed to visualize the flow patterns (plug, slug and churn flows) occurring in the mini tube by means of high-speed imaging. The values of the wall shear stress were estimated within the mini tube with the help of the gas slug velocities (8–9 m/s) and were compared with threshold shear stress values reported for the disruption of fresh <italic>E. coli</italic> cells. The results suggest that the preliminary pressurization phase may cause a substantial destabilization of the cell wall of <italic>E. coli</italic> DH5α.
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| Author: | Ratka Hoferick, Holger SchönherrORCiD, Stéphan BarbeORCiD |
|---|---|
| URN: | urn:nbn:de:hbz:832-epub4-26604 |
| DOI: | https://doi.org/10.3390/fluids9010010 |
| ISSN: | 2311-5521 |
| Parent Title (English): | Fluids |
| Publisher: | MDPI |
| Editor: | Tomoaki Kunugi |
| Document Type: | Article |
| Language: | English |
| Date of first Publication: | 2023/12/27 |
| Date of Publication (online): | 2024/05/31 |
| Tag: | Dense Phase Carbon Dioxide; Experimental Fluid Mechanics; High-Speed Imaging; Mechanical Effects; Microbial Inactivation; Multiphase Flow |
| Volume: | 9 |
| Issue: | 1 |
| Page Number: | 9 |
| Institutes: | Angewandte Naturwissenschaften (F11) |
| Dewey Decimal Classification: | 500 Naturwissenschaften und Mathematik |
| Open Access: | Open Access |
| DeepGreen: | DeepGreen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |