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Starmerella bombicola is known to produce sub‐terminally hydroxylated lactonic sophorolipids (SLs), while Candida kuoi synthesizes acidic open chain SLs with terminally hydroxylated fatty acids. Upon feeding glucose and fatty alcohols both strains form long‐chain nonionic SLs. According to structure elucidation the SLs consist of a hydroxylated fatty acid esterified with fatty alcohol and linked via a glycoside bond to the diacetylated sophorose unit. Palmityl, stearyl, and oleyl alcohols lead to products with lipid chain lengths of C32 or C36. Oleyl alcohol is the preferred substrate leading to 45 g L−1 of the double unsaturated C36 SL with S. bombicola and 20 g L−1 with C. kuoi. Scale up from shake flask to 1.5 L fermentations is possible and 65 g L−1 long‐chain SLs are obtained with S. bombicola within 7 days. Mixed feeding of oleic acid and a variety of fatty alcohols leads to new long‐chain SLs. In the presence of oleic acid the yeasts do not oxidize the fatty alcohol and thus the production of biosurfactants with tailored chain length is possible. The long‐chain SLs show good emulsification ability of water/paraffin oil mixtures at low energy input and reduced interfacial tension significantly.
Practical Applications: Sophorolipids are produced by fermentation on industrial scale focusing on cleaning and detergent applications. Mainly lactonic or anionic open‐chain forms are used today. The new long‐chain SLs presented in this manuscript are accessible with existing production technology and can be produced with high titers from cost‐efficient renewable raw materials. In contrast to the commercial products the long‐chain SLs are more hydrophobic and exhibit a strong emulsification behavior. Therefore they have the potential to broaden the application range of SLs in future. They may be useful as novel emulsifiers for cosmetic creams and lotions, pharmaceutical ointments and food products or may find application in oil spill remediation.
Urbanization processes are one of the main factors for habitat loss and fragmentation, driving global biodiversity loss and species extinction. The neotropical Atlantic forest in Brazil is considered a global key biodiversity hotspot and used to be one of the most extensive forests of the Americas. Due to substantial deforestation over centuries, its landscape was transformed into a mosaic of small forest fragments surrounded by a predominantly agricultural matrix. Urban expansion and rural urbanization have created peri-urban zones, which still can harbor natural habitat remnants,
contributing to biological diversity and thus providing essential ecosystem services to urban and rural areas. The maintenance of such ecosystem services requires an understanding of the ecological processes in the ecosystem. A prerequisite for such an in-depth insight is the quantification of the underlying ecosystem functions. The ecosystem function pest control, a trophic interaction between insectivorous birds and herbivorous arthropods, was quantified in an empirical study using artificial caterpillars as prey models. This technique allows the identification of predator groups and the assessment of their predation rates. A total of 888 plasticine caterpillars were distributed at eight sites in secondary forest fragments surrounding the university campus of the federal university of São Carlos (UFScar) in peri-urban Sorocaba, southeastern Brazil. In sixteen point counts, 72 insect-eating birds, belonging to 19 species, were identified as possible artificial caterpillar attackers. Local habitat variables were measured to describe the forest vegetation structure and the landscape context. The study aimed to assess which structural components of the
forest fragments, together with the recorded bird community variables (abundance, richness, αdiversity), best explain the estimated predation rates by birds. The mean predation rate for birds was 8.25 ± 6.3 % for a reference period of eight days, representing the first quantification of the ecosystem function pest control for the study area. The three treatments of caterpillar placement heights (ground, stem: 0.5 -1.0 m, leaf: 1.5 - 2.0 m) were the best and only estimator to explain bird
predation rates. The little dense understory and ground vegetation might have facilitated the accessibility of artificial caterpillars, especially for carnivorous arthropods and birds. The detected contrast in their foraging and predation patterns suggests that arthropods and birds complement each other in their function of pest control. Bird predation rates were found to be negatively related to the vegetation structure. Thus, more open habitats, with less understory and low tree density, but high canopy cover and including dead trees were correlated with the highest predation rates and also exhibited more specialized forest-dependent bird species. This study confirms the importance of the maintenance of forest fragments in peri-urban areas, even if they are small, to preserve forest-associated birds, to contribute to the biological diversity on a broader scale, and to prevent the loss of ecosystem functions and services, mitigating some of the adverse effects of urbanization. Further investigation of the effect among the three treatments of caterpillar
placement on the predation rates is encouraged, including comparative studies among different habitat types. For future studies, it is recommended to model the avian community variables with the vegetation structure measures to predict habitat preferences of insectivorous birds. Therefore, the sampling of more units and on a bigger scale, including over a more extended period, is necessary to improve the robustness of the results, which could provide the basis for a monetary analysis of the ecosystem service pest control by birds.
The increasing public awareness of business related impacts on environmental and social well-being, be it positive or negative, are nowadays key drivers of corporate success. Internal and external business environments exert pressure and force transparency as well as responsible action in terms of resolving environmental and social impacts. In line with this, Porter and Kramer regard business behaviour that addresses societal needs and challenges, as the “new way to achieve economic success”.
However, whereas some companies “remain trapped in an out-dated approach to value creation” and restrict their CSR commitment to the compliance with governmental regulation, others proactively address stakeholder needs. By addressing these needs, companies can benefit from grasping the opportunity of enhanced cost efficiency and lowered risk or gaining competitive advantages through establishing unique value propositions, in a way that meets stakeholder demands. Apart from that, CSR performance can be seen as powerful tool to successfully influence stakeholder perceptions in terms of reputation, and thus an exceptional source of value creation. In addition to this, organisations can profit from win-win-win situations related to syncretic value creation by means of partnering with stakeholders. This, however, implies expanding the focus of cost- and risk-reduction towards increasing competitiveness through creating mutual benefits for environment, society and the economy alike.
Although a growing number of companies have recognised the advantages coming along with Corporate Social Responsibility engagement, the accompanying efforts have not been sufficiently mature yet. Due to the fact that many organizations apply a rather narrow and out-dated approach to value creation, they fail to achieve a balance between environmental, social and economic objectives. This, in turn, implies a restriction in their ability to exploit the full value creation potential that is related to CSR engagement. But even organisations applying a broader perspective of value creation, struggle to internalise the opportunities. This is mainly due the challenge of balancing and integrating economic and non-economic criteria. At the same time, organizations are facing the challenge of getting support from senior management and staff. Thus, in order to profit from adopting a ‘broad perspective’, firms must build a deep understanding of the benefits resulting from mutual value creation. However, the main challenge remains the systematic and strategy related integration of Corporate Social Responsibility strategies into core business processes. In respect to this, organizations have to take into account that “social and environment performance are almost certainly unique to each organization”. Thus, value creation in terms of CSR has to be understood as a highly complex set of cause-an-effect relationships among mediating variables and situational contingencies, which is too specific to rely on poorly structured and generic approaches.
In brief, organisations seem to struggle establishing an understanding that does not consider environmental, social and economic benefits as mutually exclusive. Notwithstanding, that such an understanding is established the complexity of factors influencing Corporate Social Responsibility impedes to balance between economic and non-economic criteria. Furthermore, organisations have difficulties to strategically integrate, manage and measure environmental and social performance drivers as well as outcome measures.
Stable recombinant mammalian cells are of growing importance in pharmaceutical biotechnology production scenarios for biologics such as monoclonal antibodies, growth and blood factors, cytokines and subunit vaccines. However, the establishment of recombinant producer cells using classical stable transfection of plasmid DNA is hampered by low stable gene transfer efficiencies. Consequently, subsequent selection of transgenic cells and the screening of clonal cell populations are time- and thus cost-intensive. To overcome these limitations, expression cassettes were embedded into transposon-derived donor vectors. Upon the co-transfection with transposase-encoding constructs, elevated vector copy numbers stably integrated into the genomes of the host cells are readily achieved facilitating under stringent selection pressure the establishment of cell pools characterized by sustained and high-yield recombinant protein production. Here, we discuss some aspects of transposon vector technologies, which render these vectors promising candidates for their further utilization in the production of biologics.
Mental illnesses in adolescence and young adulthood are steadily increasing. Thus,mental disorders represent an individual and societal challenge and an enormous health economicburden, creating an urgent need for research and action. Mental health problems are omnipresent inthe life of young people and the internet is the first resource, which helps them to understand theirsituation. Young people with migration background often have more difficulties accessing health careservices. Digital technologies offer an ideal opportunity for a low-threshold platform that addressesthe needs of young people. The current project “GeKo:mental” aims to design a multilingual websitefor Cologne-based adolescents and young adults that will enable them to obtain comprehensiveinformation about mental illness and health, treatment options and first contact points. To designthis website, this study aims to find out what kind of health information is needed and how itshould best be presented. Nine focus group discussions with adolescents and young adults withand without migration background (N = 68) were conducted; the focus group discussions took placeat schools, in an association for social youth work and in an cultural association, which is linkedto a mosque in Cologne, Germany. A qualitative content analysis was conducted on the gatheredmaterial. The participants reported concrete challenges and needs. The results will form the basis forthe development and design of a website.
This thesis proposes four different Augmented Reality (AR) display techniques that can overcome the problem of uncertain tracking errors. Where most other papers focus on limiting the amount of error that a user tracking system generates, this paper pro-poses visualizations that limit their impact instead. For this, four different display techniques have been developed and evaluated using criteria found in related literate. Two of the four proposed solutions consistently ranked better within these criteria. The first, Bending Words, uses a mix between spatially registered information and turn instructions to achieve that result. The second, WIM, mostly relies on context infor-mation of the building instead.
Academic search systems aid users in finding information covering specific topics of scientific interest and have evolved from early catalog-based library systems to modern web-scale systems. However, evaluating the performance of the underlying retrieval approaches remains a challenge. An increasing amount of requirements for producing accurate retrieval results have to be considered, e.g., close integration of the system’s users. Due to these requirements, small to mid-size academic search systems cannot evaluate their retrieval system in-house. Evaluation infrastructures for shared tasks alleviate this situation. They allow researchers to experiment with retrieval approaches in specific search and recommendation scenarios without building their own infrastructure. In this paper, we elaborate on the benefits and shortcomings of four state-of-the-art evaluation infrastructures on search and recommendation tasks concerning the following requirements: support for online and offline evaluations, domain specificity of shared tasks, and reproducibility of experiments and results. In addition, we introduce an evaluation infrastructure concept design aiming at reducing the shortcomings in shared tasks for search and recommender systems.
Polyimides rank among the most heat-resistant polymers and find application in a variety of fields, including transportation, electronics, and membrane technology. The aim of this work is to study the structural, thermal, mechanical, and gas permeation properties of polyimide based nanocomposite membranes in flat sheet configuration. For this purpose, numerous advanced techniques such as atomic force microscopy (AFM), SEM, TEM, TGA, FT-IR, tensile strength, elongation test, and gas permeability measurements were carried out. In particular, BTDA–TDI/MDI (P84) co-polyimide was used as the matrix of the studied membranes, whereas multi-wall carbon nanotubes were employed as filler material at concentrations of up to 5 wt.% All studied films were prepared by the dry-cast process resulting in non-porous films of about 30–50 μm of thickness. An optimum filler concentration of 2 wt.% was estimated. At this concentration, both thermal and mechanical properties of the prepared membranes were improved, and the highest gas permeability values were also obtained. Finally, gas permeability experiments were carried out at 25, 50, and 100 ◦C with seven different pure gases. The results revealed that the uniform carbon nanotubes dispersion lead to enhanced gas permeation properties.
Emergency management services, such as firefighting, rescue teams and ambulances,are all heavily reliant on road networks. However, even for highly industrialised countries such asGermany, and even for large cities, spatial planning tools are lacking for road network interruptionsof emergency services. Moreover, dependencies of emergency management expand not only onroads but on many other systemic interrelations, such as blockages of bridges. The first challenge thispaper addresses is the development of a novel assessment that captures systemic interrelations ofcritical services and their dependencies explicitly designed to the needs of the emergency services.This aligns with a second challenge: capturing system nodes and areas around road networksand their geographical interrelation. System nodes, road links and city areas are integrated into aspatial grid of tessellated hexagons (also referred to as tiles) with geographical information systems.The hexagonal grid is designed to provide a simple map visualisation for emergency planners andfire brigades. Travel time planning is then optimised for accessing city areas in need by weighingimpaired areas of past events based on operational incidents. The model is developed and testedwith official incident data for the city of Cologne, Germany, and will help emergency managers tobetter device planning of resources based on this novel identification method of critical areas.
Habitat loss due to land use and land cover change (LUCC) has been identified as the main cause of global environmental change, responsible for biodiversity decline and the deterioration of ecological processes. Habitat loss and fragmentation have been driven by
processes of LUCC such as deforestation, agricultural expansion and intensification, urbanization, and globalization. The objective of this research was to determine the effects of LUCC on the process of habitat loss and the patterns of fragmentation in the surrounding landscape of the Pacuare Reserve (PR) in the Caribbean lowlands of Costa Rica. The PR is a protected area of 800 ha surrounded by an agricultural landscape with a history of over 150 years of bananas monocultures. Landsat satellite images from 1978 to 2020 were used to conduct a temporal analysis of LUCC around the PR. Patterns of change were explored using landscape metrics from the land classification images. To explore potential connectivity routes, the least cost path analysis was used to connect the PR to other protected areas. Overall, forest cover decreased in the study area at a rate of -4.8% per year during the period of 1992-1997. In the year 2001 it reached its lowest cover and then increased at a mean annual rate of 1.6%. A mean overall accuracy of 92% was obtained for the land classification process. A clear fragmentation process was observed, as shown by a decreased in forest mean patch area and largest patch index and by the increase in patch density. Although forest cover increased in the last decade, fragmentation metrics suggest this recover happened in a spatially scattered manner, due to agricultural land abandonment. Connectivity maps showed the importance of forest fragments and of the already established biological corridors for the movement of species to and from the PR, however it also evidenced the lack of connectivity between the coastal forest fragments and further inside the country located protected areas, as well as the need to promote reforestation projects, particularly between fragments of the corridors identified.
Campylobacter spp. are one of the most important food-borne pathogens, which are quite susceptible to environmental or technological stressors compared to other zoonotic bacteria. This might be due to the lack of many stress response mechanisms described in other bacteria. Nevertheless, Campylobacter is able to survive in the environment and food products. Although some aspects of the heat stress response in Campylobacter jejuni are already known, information about the stress response in other Campylobacter species are still scarce. In this study, the stress response of Campylobacter coli and Campylobacter lari to elevated temperatures (46°C) was investigated by survival assays and whole transcriptome analysis. None of the strains survived at 46°C for more than 8 h and approximately 20% of the genes of C. coli RM2228 and C. lari RM2100 were differentially expressed. The transcriptomic profiles showed enhanced gene expression of several chaperones like dnaK, groES, groEL, and clpB in both strains, indicating a general involvement in the heat stress response within the Campylobacter species. However, the pronounced differences in the expression pattern between C. coli and C. lari suggest that stress response mechanisms described for one Campylobacter species might be not necessarily transferable to other Campylobacter species.
This research analyzes the effects that eco-labels have on the demand for organic (Bio) and Fairtrade (FT) food products. The thesis also discusses the individual determinants and motivations behind those effects. The analysis builds on data obtained from a self-programmed and self-conducted survey, with a sample of 869 students from different universities of Cologne, Germany. The Bio/FT preference is measured experimentally by randomly assigning individuals to treatment and control groups. The experiment simulates life decisions using actual pictures and prices of four products: packed and processed spaghetti, fresh tomatoes, packed raw meat, and packed orange juice.
The existence, size, and direction of statistically significant eco-label effects were obtained with two sample tests of proportions. The results prove that the FT label has a positive differential effect on consumer’s demand. The presence of the FT label makes the purchase of this juice 9.1% higher than other juices not labeled as FT. This finding confirms the hypothesis that eco-labels have a positive effect on sustainable consumption. A surprising finding is that the presence of the Bio label lowers the purchase of organic pasta and tomatoes 7.7% and 9.4% respectively. This finding is interesting because it suggests that Bio labels are not driving the demand for sustainable tomatoes or pasta for this population. Regional and cheaper alternatives are preferred by consumers in this cases.
The motivations behind consumer choices of different options were thoroughly analyzed. Binomial logistic regressions and qualitative text analysis show that the variance in the intention to consume eco-labeled food is explained mainly by price concerns and attitudes about value for money, but also by the influence of life partners as shopping referents, and the perceived behavioral controls of time and ability to monitor compliance of label standards, thus trust them.
The final remarks support the use of the Fairtrade eco-label as a market-based instrument to guide sustainable food consumption among young adults in this context, and propose changes that could make the Bio label more attractive for the targeted population. The thesis demonstrates which individual factors should be inevitably considered when implementing labeling to foster sustainable consumption. Hence, it is useful for evaluations of public and private certification schemes, and for companies that support sustainable food markets. Projects looking to understand and drive sustainable production and consumption decisions should consider this reading.
We examine the impact of the existence on an explicit deposit insurance (DI) scheme and its design features on bilateral cross‐border deposits (CBD) in a gravity model setting. We find that both the absolute quality of a country's DI and its relative quality vis‐à‐vis other countries' DI generally affect depositor behavior. However, during systemic banking crises, cross‐border depositors primarily seek countries with the best DI schemes. Similarly, during the 2008–2009 great financial crisis, the emergency actions taken by the governments, which supply and maintain these safe havens, have led to substantial relocations of CBD. (JEL F34, G18)
Wetlands offer different ecosystem services that contribute to human well-being (Kovács et al., 2015). According to the Ramsar Convention Secretariat (2018) wetland located in urban areas have been threatened by several activities such as drainage, pollution, encroachment, agriculture, among others. On the other hand, wetland degradation reduces the resilience of hazards like floods and storm surges (Kumar et al., 2017). For that reason, ecosystem-based disaster risk reduction (Eco-DRR) is an important strategy which enhances the conservation and restoration of ecosystems to reduce disaster risk aiming to sustainable development and resilience (Estrella & Saalismaa, 2013). Despite international recognition of the importance of wetlands, urban wetlands have diminished their capacity to cope with flood threats (Boyer and Polasky, 2006) due to the aforementioned human impacts.
That is why this thesis aimed to identify the role of urban wetlands in Bogota, Colombia, that has an urban wetland complex that is recognised as a Ramsar site in 2018. However, wetlands in the city reduced its area from 50.000 hectares to less than 800, approximately, in less than 40 years, mainly because of urban expansion and encroachment (IDIGER, 2018). To achieve this objective, an analysis of the city’s risk management framework was conducted, as well as a stakeholder analysis based on semi-structured interviews and a spatial-temporal analysis for the period 1998-2017, for which the Jaboque wetland was used as a case study. This wetland is located near the Bogotá River and is in the area threatened by flooding.
It was possible to determine that national and district policies on wetlands, biodiversity, and climate change adaptation address some ecosystem functions. Still, disaster risk reduction is not strongly linked to them. Thus, based on the case study, the wetlands in Bogota have not played a decisive role in flood risk management in the city.
The use of nematic liquid crystal (LC) mixtures for microwave frequency applicationspresents a fundamental drawback: many of these mixtures have not been properly characterizedat these frequencies, and researchers do not have an a priori clear idea of which behavior they canexpect. This work is focused on developing a new procedure for the extraction of the main parametersof a nematic liquid crystal: dielectric permittivity and loss tangent at 11 GHz under differentpolarization voltages; splay elastic constantK11, which allows calculation of the threshold voltage(Vth); and rotational viscosityγ11, which allows calculating the response time of any arbitrary device.These properties will be calculated by using a resonator-based method, which is implementedwith a new topology of substrate integrated transmission line. The LC molecules should be rotated(polarized) by applying an electric field in order to extract the characteristic parameters; thus,the transmission line needs to have two conductors and low electric losses in order to preserve theintegrity of the measurements. This method was applied to a well-known liquid crystal mixture(GT3-23002 from MERCK) obtaining the permittivity and loss tangent versus bias voltage curves,the splay elastic constant, and the rotational viscosity of the mixture. The results validate the viabilityof the proposed method.
Proper satellite-based crop monitoring applications at the farm-level often require near-daily imagery at medium to high spatial resolution. The combination of data from different ongoing satellite missions Sentinel 2 (ESA) and Landsat 7/8 (NASA) provides this unprecedented opportunity at a global scale; however, this is rarely implemented because these procedures are data demanding and computationally intensive. This study developed a robust stream processing for the harmonization of Landsat 7, Landsat 8 and Sentinel 2 in the Google Earth Engine cloud platform, connecting the benefit of coherent data structure, built-in functions and computational power in the Google Cloud. The harmonized surface reflectance images were generated for two agricultural schemes in Bekaa (Lebanon) and Ninh Thuan (Vietnam) during 2018–2019. We evaluated the performance of several pre-processing steps needed for the harmonization including the image co-registration,
Bidirectional Reflectance Distribution Functions correction, topographic correction, and band adjustment. We found that the misregistration between Landsat 8 and Sentinel 2 images varied from 10 m in Ninh Thuan (Vietnam) to 32 m in Bekaa (Lebanon), and posed a great impact on the quality of the final harmonized data set if not treated. Analysis of a pair of overlapped L8-S2 images over the Bekaa region showed that, after the harmonization, all band-to-band spatial correlations were greatly improved. Finally, we demonstrated an application of the dense harmonized data set for crop mapping and monitoring. An harmonic (Fourier) analysis was applied to fit the detected unimodal, bimodal and trimodal shapes in the temporal NDVI patterns during one crop year in Ninh Thuan province. The derived phase and amplitude values of the crop cycles were combined with max-NDVI as an R-G-B false composite image. The final image was able to highlight croplands in bright colors (high phase and amplitude), while the non-crop areas were shown with grey/dark (low phase and amplitude). The harmonized data sets (with 30 m spatial resolution) along with the Google Earth Engine scripts used are provided for public use.
This thesis analyzes the history and current circumstances women belonging to ethnic minorities find themselves in. It also examines how social work deals with this issue. The situation of Indigenous women in Canada as well as Sinti and Roma women in Germany will serve as an example. Within Germany, there are no comparable Indigenous groups that went through a history similar to that of the Indigenous people of Canada. Thus, within the realms of possibility, it is intended to compare their situation to that of Sinti and Roma women and their communities in Germany. After comparing both groups and their current situation, possible consequences and improvements for the field of social work will be indicated
Usage of smart home solutions implies generation, processing, and storage of machine and personal data. Recently made public cases of data breaches and misuse increased peoples’ concerns of data security and decreased the trust in secure data handling and smart home technologies. Hence potential benefits are not exploited. It is therefore necessary to analyse how manufacturers can increase their online trust perception.
Requirements of (potential) customers of web based services for HVAC systems are identified using thematic analysis for 23 conducted interviews using online trust perception literature as basis. Manufacturer independent websites are derived as the most important online touch point for customers with manufacturers and products. Determined content and structure measures for online touch points managed by manufacturers derived to positively influence the manufacturer and manufacturer independent touch points regarding perception of trust. The derived trust concept must be evaluated in the following using the defined evaluation plan.
Against the background of a worldwide decrease in the number of gauging stations,the estimation of river discharge using spaceborne data is crucial for hydrological research, rivermonitoring, and water resource management. Based on the at-many-stations hydraulic geometry(AMHG) concept, a novel approach is introduced for estimating river discharge using Sentinel-1time series within an automated workflow. By using a novel decile thresholding method, no a prioriknowledge of the AMHG function or proxy is used, as proposed in previous literature. With arelative root mean square error (RRMSE) of 19.5% for the whole period and a RRMSE of 15.8%considering only dry seasons, our method is a significant improvement relative to the optimizedAMHG method, achieving 38.5% and 34.5%, respectively. As the novel approach is embedded intoan automated workflow, it enables a global application for river discharge estimation using solelyremote sensing data. Starting with the mapping of river reaches, which have large differences inriver width overthe year, continuous river width time series are created using high-resolution andweather-independent SAR imaging. It is applied on a 28 km long section of the Mekong River nearVientiane, Laos, for the period from 2015 to 2018.