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Starmerella bombicola is known to produce sub‐terminally hydroxylated lactonic sophorolipids (SLs), while Candida kuoi synthesizes acidic open chain SLs with terminally hydroxylated fatty acids. Upon feeding glucose and fatty alcohols both strains form long‐chain nonionic SLs. According to structure elucidation the SLs consist of a hydroxylated fatty acid esterified with fatty alcohol and linked via a glycoside bond to the diacetylated sophorose unit. Palmityl, stearyl, and oleyl alcohols lead to products with lipid chain lengths of C32 or C36. Oleyl alcohol is the preferred substrate leading to 45 g L−1 of the double unsaturated C36 SL with S. bombicola and 20 g L−1 with C. kuoi. Scale up from shake flask to 1.5 L fermentations is possible and 65 g L−1 long‐chain SLs are obtained with S. bombicola within 7 days. Mixed feeding of oleic acid and a variety of fatty alcohols leads to new long‐chain SLs. In the presence of oleic acid the yeasts do not oxidize the fatty alcohol and thus the production of biosurfactants with tailored chain length is possible. The long‐chain SLs show good emulsification ability of water/paraffin oil mixtures at low energy input and reduced interfacial tension significantly.
Practical Applications: Sophorolipids are produced by fermentation on industrial scale focusing on cleaning and detergent applications. Mainly lactonic or anionic open‐chain forms are used today. The new long‐chain SLs presented in this manuscript are accessible with existing production technology and can be produced with high titers from cost‐efficient renewable raw materials. In contrast to the commercial products the long‐chain SLs are more hydrophobic and exhibit a strong emulsification behavior. Therefore they have the potential to broaden the application range of SLs in future. They may be useful as novel emulsifiers for cosmetic creams and lotions, pharmaceutical ointments and food products or may find application in oil spill remediation.
Stable recombinant mammalian cells are of growing importance in pharmaceutical biotechnology production scenarios for biologics such as monoclonal antibodies, growth and blood factors, cytokines and subunit vaccines. However, the establishment of recombinant producer cells using classical stable transfection of plasmid DNA is hampered by low stable gene transfer efficiencies. Consequently, subsequent selection of transgenic cells and the screening of clonal cell populations are time- and thus cost-intensive. To overcome these limitations, expression cassettes were embedded into transposon-derived donor vectors. Upon the co-transfection with transposase-encoding constructs, elevated vector copy numbers stably integrated into the genomes of the host cells are readily achieved facilitating under stringent selection pressure the establishment of cell pools characterized by sustained and high-yield recombinant protein production. Here, we discuss some aspects of transposon vector technologies, which render these vectors promising candidates for their further utilization in the production of biologics.
Mental illnesses in adolescence and young adulthood are steadily increasing. Thus,mental disorders represent an individual and societal challenge and an enormous health economicburden, creating an urgent need for research and action. Mental health problems are omnipresent inthe life of young people and the internet is the first resource, which helps them to understand theirsituation. Young people with migration background often have more difficulties accessing health careservices. Digital technologies offer an ideal opportunity for a low-threshold platform that addressesthe needs of young people. The current project “GeKo:mental” aims to design a multilingual websitefor Cologne-based adolescents and young adults that will enable them to obtain comprehensiveinformation about mental illness and health, treatment options and first contact points. To designthis website, this study aims to find out what kind of health information is needed and how itshould best be presented. Nine focus group discussions with adolescents and young adults withand without migration background (N = 68) were conducted; the focus group discussions took placeat schools, in an association for social youth work and in an cultural association, which is linkedto a mosque in Cologne, Germany. A qualitative content analysis was conducted on the gatheredmaterial. The participants reported concrete challenges and needs. The results will form the basis forthe development and design of a website.
Academic search systems aid users in finding information covering specific topics of scientific interest and have evolved from early catalog-based library systems to modern web-scale systems. However, evaluating the performance of the underlying retrieval approaches remains a challenge. An increasing amount of requirements for producing accurate retrieval results have to be considered, e.g., close integration of the system’s users. Due to these requirements, small to mid-size academic search systems cannot evaluate their retrieval system in-house. Evaluation infrastructures for shared tasks alleviate this situation. They allow researchers to experiment with retrieval approaches in specific search and recommendation scenarios without building their own infrastructure. In this paper, we elaborate on the benefits and shortcomings of four state-of-the-art evaluation infrastructures on search and recommendation tasks concerning the following requirements: support for online and offline evaluations, domain specificity of shared tasks, and reproducibility of experiments and results. In addition, we introduce an evaluation infrastructure concept design aiming at reducing the shortcomings in shared tasks for search and recommender systems.
Editorial
(2020)
Polyimides rank among the most heat-resistant polymers and find application in a variety of fields, including transportation, electronics, and membrane technology. The aim of this work is to study the structural, thermal, mechanical, and gas permeation properties of polyimide based nanocomposite membranes in flat sheet configuration. For this purpose, numerous advanced techniques such as atomic force microscopy (AFM), SEM, TEM, TGA, FT-IR, tensile strength, elongation test, and gas permeability measurements were carried out. In particular, BTDA–TDI/MDI (P84) co-polyimide was used as the matrix of the studied membranes, whereas multi-wall carbon nanotubes were employed as filler material at concentrations of up to 5 wt.% All studied films were prepared by the dry-cast process resulting in non-porous films of about 30–50 μm of thickness. An optimum filler concentration of 2 wt.% was estimated. At this concentration, both thermal and mechanical properties of the prepared membranes were improved, and the highest gas permeability values were also obtained. Finally, gas permeability experiments were carried out at 25, 50, and 100 ◦C with seven different pure gases. The results revealed that the uniform carbon nanotubes dispersion lead to enhanced gas permeation properties.
Emergency management services, such as firefighting, rescue teams and ambulances,are all heavily reliant on road networks. However, even for highly industrialised countries such asGermany, and even for large cities, spatial planning tools are lacking for road network interruptionsof emergency services. Moreover, dependencies of emergency management expand not only onroads but on many other systemic interrelations, such as blockages of bridges. The first challenge thispaper addresses is the development of a novel assessment that captures systemic interrelations ofcritical services and their dependencies explicitly designed to the needs of the emergency services.This aligns with a second challenge: capturing system nodes and areas around road networksand their geographical interrelation. System nodes, road links and city areas are integrated into aspatial grid of tessellated hexagons (also referred to as tiles) with geographical information systems.The hexagonal grid is designed to provide a simple map visualisation for emergency planners andfire brigades. Travel time planning is then optimised for accessing city areas in need by weighingimpaired areas of past events based on operational incidents. The model is developed and testedwith official incident data for the city of Cologne, Germany, and will help emergency managers tobetter device planning of resources based on this novel identification method of critical areas.
Campylobacter spp. are one of the most important food-borne pathogens, which are quite susceptible to environmental or technological stressors compared to other zoonotic bacteria. This might be due to the lack of many stress response mechanisms described in other bacteria. Nevertheless, Campylobacter is able to survive in the environment and food products. Although some aspects of the heat stress response in Campylobacter jejuni are already known, information about the stress response in other Campylobacter species are still scarce. In this study, the stress response of Campylobacter coli and Campylobacter lari to elevated temperatures (46°C) was investigated by survival assays and whole transcriptome analysis. None of the strains survived at 46°C for more than 8 h and approximately 20% of the genes of C. coli RM2228 and C. lari RM2100 were differentially expressed. The transcriptomic profiles showed enhanced gene expression of several chaperones like dnaK, groES, groEL, and clpB in both strains, indicating a general involvement in the heat stress response within the Campylobacter species. However, the pronounced differences in the expression pattern between C. coli and C. lari suggest that stress response mechanisms described for one Campylobacter species might be not necessarily transferable to other Campylobacter species.
We examine the impact of the existence on an explicit deposit insurance (DI) scheme and its design features on bilateral cross‐border deposits (CBD) in a gravity model setting. We find that both the absolute quality of a country's DI and its relative quality vis‐à‐vis other countries' DI generally affect depositor behavior. However, during systemic banking crises, cross‐border depositors primarily seek countries with the best DI schemes. Similarly, during the 2008–2009 great financial crisis, the emergency actions taken by the governments, which supply and maintain these safe havens, have led to substantial relocations of CBD. (JEL F34, G18)
The use of nematic liquid crystal (LC) mixtures for microwave frequency applicationspresents a fundamental drawback: many of these mixtures have not been properly characterizedat these frequencies, and researchers do not have an a priori clear idea of which behavior they canexpect. This work is focused on developing a new procedure for the extraction of the main parametersof a nematic liquid crystal: dielectric permittivity and loss tangent at 11 GHz under differentpolarization voltages; splay elastic constantK11, which allows calculation of the threshold voltage(Vth); and rotational viscosityγ11, which allows calculating the response time of any arbitrary device.These properties will be calculated by using a resonator-based method, which is implementedwith a new topology of substrate integrated transmission line. The LC molecules should be rotated(polarized) by applying an electric field in order to extract the characteristic parameters; thus,the transmission line needs to have two conductors and low electric losses in order to preserve theintegrity of the measurements. This method was applied to a well-known liquid crystal mixture(GT3-23002 from MERCK) obtaining the permittivity and loss tangent versus bias voltage curves,the splay elastic constant, and the rotational viscosity of the mixture. The results validate the viabilityof the proposed method.