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Online services such as social networks, online shops, and search engines deliver different content to users depending on their location, browsing history, or client device. Since these services have a major influence on opinion forming, understanding their behavior from a social science perspective is of greatest importance. In addition, technical aspects of services such as security or privacy are becoming more and more relevant for users, providers, and researchers. Due to the lack of essential data sets, automatic black box testing of online services is currently the only way for researchers to investigate these services in a methodical and reproducible manner. However, automatic black box testing of online services is difficult since many of them try to detect and block automated requests to prevent bots from accessing them.
In this paper, we introduce a testing tool that allows researchers to create and automatically run experiments for exploratory studies of online services. The testing tool performs programmed user interactions in such a manner that it can hardly be distinguished from a human user. To evaluate our tool, we conducted - among other things - a large-scale research study on Risk-based Authentication (RBA), which required human-like behavior from the client. We were able to circumvent the bot detection of the investigated online services with the experiments. As this demonstrates the potential of the presented testing tool, it remains to the responsibility of its users to balance the conflicting interests between researchers and service providers as well as to check whether their research programs remain undetected.
STEPsCON 2018 was jointly organized by the Faculty of Applied Natural Sciences of TH Köln (Germany) and the University of Oulu (Finland) on the occasion of the 50th anniversary of the Leverkusen – Oulu town twinning. The conference focused on sustainability issues and covered the current state of research in four key topics:
1. Sustainable Medicine and Pharmaceuticals
2. Resources and Bioremediation
3. Sustainable Chemistry & Industrial Biotechnology
4. Innovative Materials & Formulations
For use in a landfill, a laboratory reactor for safe and environmentally friendly biological utilization of low-concentration methane gas will be further developed. The current principle of denitrification-coupled aerobic methane oxidation will be replaced by methane oxidation under anaerobic conditions. Anaerobic methane oxidation offers the advantage that, in addition to methane, nitrate also undergoes biodegradation. Another advantage is that the oxygen content can be significantly lower. This reduces the risk of the formation of an explosive atmosphere in the reactor. Currently, the principle of anaerobic methane oxidation is known. However, organisms capable of doing so are not yet available as a pure culture. Therefore, several biomasses were probed for the ability of anaerobic methane oxidation. It was found that moor-heavy sediment, activated sludge from the leachate treatment plant and biomass from the local biogas plant oxidize methane after the natural carbon source (C source) was been removed.
The synthesis of 17-hydroxy-oleic acid based oligomeric esters was investigated with immobilized Pseudozyma antarctica Lipase B and hexanediol as co-substrate. The effects of different reaction parameters on velocity and product composition at equilibrium conditions were analyzed. The synthesis of oleic acid esters was used as a reference system for initial evaluation of reaction parameters. The reaction with oleic acid and hexanediol was fastest at an enzyme concentration of 5% at 60 °C and high conversions of > 90 % were achieved in non-polar solvents in the presence of molecular sieves. In heptane an oleic acid conversion of 96 % was reached with a final diester to monoester ratio of > 4:1. In syntheses trials with 17-hydroxy-oleic acid the formation of oligomers was verified with GPC, however; conversion was generally lower than with oleic acid. Removal of hydroxyl fatty acid monomers and dimers and the formation ester functionalities could be verified by GC analysis. An increase of the degree of oligomerization was observed simultaneously by GPC analysis. The number-average molecular weight was around 1400 in the best trials corresponding to a degree of oligomerization of around 4 units of hydroxyl-fatty acid attached to a hexanediol core. Though transformations were not complete, the final oligomer size was in the lower range of polyester diols used for polyurethane manufacturing.
Due to the worldwide shortage of petrochemical based resources, the usage of renewable bio-based raw materials for established and novel products becomes increasingly important.[1] Such bio-based resources are already used for the fabrication of a variety of products, e. g. paper, lubricants, detergents or cosmetics. In the future they are expected to emerge in many more applications in industry and household.[1]
A very promising approach relies on the use of glycolipids as a source of hydroxy-oleic acid.[2] Microbial glycolipids are produced for instance via fermentation from natural resources such as plant oils and sugar.[3] After fermentation complex product mixtures are obtained with the composition depending on the microorganism, substrate and fermentation time.[3] The successful use of microbial glycolipids and hydroxy-oleic acid (HOA) derived therefrom as bio-based intermediates requires reliable analytical methods as well as robust manufacturing processes for the synthesis and cleavage of bio-based molecules. In order to obtain hydroxy-oleic acids as bio-based intermediates, the acidic cleavage of microbial derived sophorolipid was investigated. In addition the implementation of HOA in polyurethane (PU) systems was explored.
The introduction of Feed-in tariffs in the German Renewable Energy Act (EEG) fuelled the growth of anaerobic digestion (AD) industry making Germany the country with highest number of operational AD plants. However, the rapid expansion of AD industry resulted in some unwanted side-effects such as food vs fuel debate, increased prices for electricity and the temporal mismatch between supply and demand of electricity grid. Subsequent amendments in EEG has tried to address some of these issues by reduction in Feed-in tariffs, introduction of a cap on cereal based feedstocks and providing premium for energy production in accordance with market demand. Furthermore, the Feed-in tariffs which were introduced for 20 years are soon going to expire. The changes in legal and political discourse is soon going to introduce some new challanges to the AD industry. This paper has discussed some of these challanges and their potential solutions.
The electricity network is undergoing a change due to reducing costs for renewable energy sources. Subsidy programs for renewable energy sources are changing and the funding available is being reduced. This will have an impact on anaerobic digestion which in some cases may struggle financially. Overfeeding is one of the most common mechanisms of inhibition in the process, and by shifting to intermittent feeding for on-demand production, this change will provide more information about the digestion process and could be used to detect the beginning of inhibition due to overfeeding. This paper discusses the shift towards intermittent production and how this change can be used to monitor the anaerobic digestion process.
Comparative analysis of non-natural acceptor glucosylation with sucrase enzymes of family GH 70
(2019)
Mutan- and alternansucrase were analyzed for their non-natural glucosylation potential with catecholic compounds caffeic acid and nordihydroguaiaretic acid (NDGA) as well as with non-catecolic p-coumaric acid and umbellic acid. Mutansucrase accepted both catecholic substrates and high glucosylation yields of 92 % with caffeic acid and 81 % with NDGA were obtained. The enzyme showed a clear regio-preference for the catechol 4-OH, which corresponds to findings from our previous work with Leuconostoc and Weissella derived glucansucrases. The substrate spectrum of the alternansucrase was broader and all substrates were successfully glucosylated with a preference for the catechols. Interestingly alternansucrase possessed a different regio-specificity. With caffeic acid the 3-O-α-D-glucoside was the major product. A similar substrate spectrum and regioselectivity pattern was observed in previous glucansucrase screenings only with glucansucrase from strain Weissella beninensis DSM 22752. Therefore it may be concluded that the W. beninensis enzyme is an alternansucrase type enzyme as well.
In the degradation of ammonia (NH4+) to gaseous nitrogen (N2), the nitrification is one of the two reaction steps. The nitrification itself is divided in two steps and is performed by two different types of bacteria. Current literature has shown that there are types of bacteria, which have the genetic equipment to perform both steps in one bacteria. Nevertheless, in wastewater and landfill leachate treatment, ammonia-oxidizing organisms (AOO) and nitrite-oxidizing organisms (NOO) occur as a symbiosis. The intermediate of the two consecutive reaction steps (NO2-, nitrite) is toxic. For this reason, both steps are necessary for the two bacterial groups. To determine the ratio of AOO, NOO and heterotrophic bacteria (which use organic compounds as carbon and energy source) the oxygen uptake rate (OUR) with selective inhibition with N-allylthiourea (ATU) and azide is used. In the inflow of a pilot plant in one street a step by step increased amount of a process water out of a fermentation plant was added to the landfill leachate. For comparison, the other street was supplied only with landfill leachate with the same amount of nitrogen. As a result, comparable values for the different bacterial groups and reproducible results were measured and lead to a better understanding of the analysed nitrification sludge. Deeper understanding of the behavior of the different groups will result in a reduce risk of malfunctions and a more stable operation in the wastewater or landfill leachate treatment plant.
The whole site of the waste disposal centre Leppe in Lindlar has been modified by the project :metabolon into an authentic learning site for knowledge transfer. Addressing all age groups, the project offers insights into environmental knowledge and explains contexts of resources and material flows. The site conditions allow practical outlooks on future energy systems. Following the meta theme of “lifetime learning”, pupils and students are addressed by different modules, considering their individual learning levels.
Aerobic microbial cultivations are industrially important group of processes and pose challenges for the reactor design. In particular, estimation of industrial scale conditions is difficult from laboratory and pilot scale data. Due to complex interaction of gas/liquid phase hydrodynamics, mass transfer parameters and microbial metabolism, both improvement of modelling tools and reactor design are desired. We present an approach to estimate growth conditions in industrial scale reactor by combining black-box metabolic models with CFD-model.
The reactor type used here is Outotec OKTOP9000®, which is used in the industrial hydrometallurgical processes at 900 m3 scale. It is adopted to a laboratory setting and compared to stirred tank reactor (STR) in gas dispersion, mass transfer and yeast cultivation experiments. In addition, a kinetic model for the yeast growth is developed based on literature sources and validated by the laboratory scale batch cultivations. This kinetic model is used along with CFD-model that is developed to describe the flow and mass transfer conditions in the industrial scale reactor.
The laboratory scale experiments show the feasibility of OKTOP9000® reactor when compared to STR, particularly with improved gas handling capacity. The modelling approach shows qualitatively similar behavior in the large scale simulations when compared to laboratory scale cultivations.
Before transporting the landfill leachate to municipal wastewater treatment plant it has to be treated in a landfill leachate treatment plant, as it comprises high concentrations of ammonium. The elimination of ammonium load in the leachate is usually done by the combined processes of nitrification and denitrification with a specially adapted biocenosis in the activated sludge (AS). For each of the steps, specialized bacteria such as Nitrosomonas, Nitrobacter and Paracoccus are used to transfer the ammonia to gaseous nitrogen. The aim of this investigation was to find suitable process parameters for a complementary treatment of fermentation water from a biogas plant together with landfill leachate. The processed water of the biogas plant consists of a higher concentration of ammonium and carbon sources or easily degradable volatile fatty acids. It can save the usage of external carbon source (acetic acid) and additionally it could also compensate the missing volumes of leachate in times of low rain and low leachate flows. To maintain the high workload for the existing leachate treatment pilot plant (LTPP), a combined treatment of landfill leachate and process water is also of economic and of ecological interest. The long-term adaption process of the biocenosis needs to be done step-by-step. Innovative process monitoring is needed to prevent biocenosis collapse. In our study, we present our set-up, a closer look at the ongoing experiment and the long-term changes in the biocenosis.
Pseudozyma antarctica Lipase B catalyzed esterification and transesterification in deep eutectic solvents (DES) was investigated in reaction systems with alcohols of different polarity. Coconut oil and crude biodiesel were deacidified successfully with non-immobilized CALBL and final acid values of 1.2 for biodiesel and 0.5 for coconut oil were obtained, while no esterification with ethanol was observed without DES. Water depletion of the lipid phase in the presence of water adsorbing DES causes this difference. Analysis of water contents revealed a 10 fold lower water content of the lipid phase in the presence of a second DES phase than in trials without utilization of DES. In contrast reactions of hydrophilic polyols are suppressed in the presence of DES. While the esterification of fructose and the transesterification with glycerol worked well in the polar solvent 2-methyl-2-butanol, almost no fructose esterification and a decreased transesterification with glycerol were observed in the presence of DES. Analysis of logP values of the substrates explains the substrate dependent differences in reactivity. The polar alcohols are probably bound strongly in the hydrogen-bonding network of the DES phase and are thus not available for lipase catalyzed reactions.
Modern industrial biomass combustion plants are regulated by the power and/or combustion control. In this process, the implemented sensors collect the relevant measured data. The aim is to achieve ideal combustion with optimum efficiency and to minimize gas emissions. For this purpose, a group within the research project Metabolon developed new regulatory procedures in order to record the combustion process of a biomass combustion plant using a webcam. The recordings were evaluated automatically and were used for a better monitoring of the process. In addition, the webcam-based method aims, among other things, to provide private homes with a cost-effective variant as an alternative to industrial system solutions.
In the last few decades raw material molasses, used in large scale fermentations in the production of bioethanol, citric acid, (baker´s) yeast and yeast extracts, has become more and more expensive. That is why agro-industrial wastes have become an interesting alternative. They are being produced in large volumes every day and represent a serious environmental problem considering its high organic content. The present contribution aims to demonstrate how waste products of wine production can be employed as substrate in bioethanol production. Cultivation of yeast and bioethanol production on molasses and grape pomace extract was studied in flasks in laboratory scale. This work should be regarded as an example of integrated sustainability which demonstrates how the waste from one industrial process is used as feedstock for another.
The southeast of Córdoba province used to be originally covered by hundreds of wetlands that got heavily modified or drained in the last few decades. Since wetlands provide various important ecosystem services (ESS) for human well-being, their degradation created several problems in La Picasa basin, among which floods are the most obvious one. The wise use of wetlands is increasingly acknowledged to be part of nature-based solution approaches reducing disaster risk. However, in the study area these approaches remain a relatively new concept to decision makers and the lack of knowledge on their effectiveness and implementation process poses a serious barrier to their adoption.
To overcome this obstacle, this dissertation applies an ESS perspective on the current problems of La Picasa basin and sets it in a context of socio-ecological system (SES) theory. A comprehensive analysis of (1) the role wetlands have played in the historic development of the SES, (2) important stakeholder dynamics that create opportunities or restrictions for the conservation of wetlands and (3) possible management approaches to inverse negative ESS trade-offs and feedback loops, was performed.
Results demonstrate that the current problems of floods have both natural and anthropogenic causes. In this regard, wetlands hold a vital role in the complex historic interactions between the social and ecological drivers of changes in the water balance. Although a social network between stakeholders exists, several conflicts prevent a proper functioning of a basin-wide integrated management concept based on wetland restoration. Nature-based solution approaches, putting wetlands in the center of attention of future management strategies, were found to hold a high potential to reduce the risk of floods and, as a side-effect boost biodiversity and habitat quality in the study area.
In the Mesoamerican forest Selva Maya, multiple driving forces create an imbalance in the sensitive human-nature relation and demand for innovative management strategies for its re-establishment. Within the Guatemalan Maya Biosphere Reserve (MBR), core areas are under strict protective legislation and agricultural activity is permitted only within a bordering buffer zone (BZ), which covers great part of the Guatemalan department Petén. Here, the implementation of agroecological practices by multiple stakeholders aims at tackling the principle driving forces of environmental degradation and thus at reducing the pressure on Central America’s largest tropical forest area. Since 2011, the Deutsche Gesellschaft für Internationale Zusammenarbeit (GIZ) has assisted local stakeholders by carrying out the project “Conservation and sustainable use of the Selva Maya”. This project has offered technical support, cooperated with national institutions, and assisted multiple target groups to nudge agroecological transitions at the household and community level. As the establishment of agroecological systems face main obstacles stemming from the socio-ecological setting of the respective area, the following work presents a context specific analysis for the adaption of established strategies in the MBR BZ. Therefore, it raises the following research questions: What are the current properties of the socio-ecological system that describes the BZ? How has the GIZ’s project nudged and guided agroecological transitions? Which factors have favored or limited the turn to agroecological farming? And finally: Which recommendations derive for the navigation of agroecological transitions? The overall research approach is orientated on the framework of ecosystem stewardship1 and incorporates elements of system theory and resilience science. The framework has been adapted by combining two approaches on different management levels. The social-ecological system approach2 is used to describe the socio-ecological system of the BZ, while the evaluation of the pilot groups‘ AESs follows the Mexican MESMIS3 approach for sustainability assessments. By the integration of both approaches, it is revealed that the socio-economic context impedes or hinders the implementation of agroecological strategies for the majority of farmers. The application of the MESMIS framework has revealed that the installed monitoring mechanism is dysfunctional. Findings further indicate that there is potential for transitions of individual AESs, but they demand investments and support with the current circumstances of reducing farmers’ vulnerability. The rapidly decreasing social and environmental conditions for family farmers in the BZ are most likely not addressed by solutions that the agroecological approach tackles. Recommendations for the immediate improvement of the strategy include adjustments of the project’s proceedings as well as fundamental changes in conservation paradigm and governance to maintain the necessary functionality of the socio-ecological system.
The ‘Energy Crisis’ has become the talk of the town in pretty much every developing and lower developing countries in today’s world. It is characterized by a state where the country’s locally available energy resources are being depleted and it is dependent on imported fuel. The problem is considered as although not parallel, but a descendant of the food crisis in terms of the seriousness of the problems in developing nations essentially in Sub-Saharan Africa (SSA). Ethiopia is one such country which nevertheless going through a rapid scale of development (nearly 11 % annual growth rate as of 2017 according to the World Bank) and also is endowed with an enormous amount of natural resources such as hydro, wind, solar, geothermal energy potential. The Ethiopian power sector is heavily dependent on the country’s hydropower resources. However, it needs to diversify its energy sector and integrate new and other renewable energy sources because, in the longer term, its extreme hydropower dependence may put its power sector vulnerable to natural risks like droughts which are very likely scenarios due to the climate change. Since the lack of access to modern forms of energy services left no choice for the Ethiopians than to continue their traditional biomass use, and it results in unsustainable environmental harm with deforestation, soil erosion, and many others. To address this issue, Ethiopia is taking necessary steps towards climate-friendly industrialization of the economy.
In order to understand this transition, a socio-technical analysis of Ethiopian ambitious transformation from an agrarian society to a climate resilient green society has been presented in this paper. An analytical framework will be formulated as a prerequisite for the study by introducing the theory of Multilevel Perspective (MLP). This theory enables the understanding of three different levels of socio-technical environment namely niches, regime, and landscape in which the respective actors interact with each other to facilitate the process of transition. As a part of laying the groundwork, this thorough analysis constitutes all the country’s energy-related activities and associated energy demands, conversion technologies, current fuel mix, primary energy resources, and energy policies in the Ethiopian energy system. The LEAP analysis results from Mr. Md Alam Mondal and group are summarized to obtain an understanding of the country’s total energy demand scenarios.
Consequently, the actors from each socio-technical level have been identified in the context of Ethiopia and their dynamics of interaction have been explained in order to understand the process of energy system transition of Ethiopia in the direction of diversification of its energy system and hence result in the expansion of new renewable energy sector. Most importantly the assessment suggests that the transition process is majorly driven by top-down forces and intra-level reconfiguration of regime actors. There are no bottom-up forces acting as only a little research and development work takes place in the country to develop new radical changes/technological niches. A developing country like Ethiopia has undoubtedly a bright future ahead with all systems in place and the nature-gifted natural resource potential. The ambitious goals set by the country and the international help from developed allies are definitely working in tandem to ensure their accomplishment. With its guiding vision towards development and the global climate change movement, Ethiopia surely has the potential to lead by example.
La Picasa basin, an interjurisdictional endorreic basin of 5282 km2 located in the “Central Pampa” in Argentina, has suffered repeated flooding caused by an increasing water level of La Picasa lagoon, affecting livelihoods, infrastructure, transportation and agriculture. Although water infrastructure has been built to regulate water excesses, it has not been effective in reducing the flood risk.
To improve the knowledge of the hydrological system, the master’s thesis aimed to develop a water balance model of La Picasa lagoon at a monthly time step between the hydrological years 2007/2008 and 2016/2017. Specifically, the objectives were to identify and quantify the most important components and processes determining its water level, area and volume and to propose hypothetical simulation scenarios based on different pumping operation schemes.
The description of the conceptual model and implementation of a sensitivity analysis allowed to identify the inflow and outflow components of the water balance and quantify their relative contributions, namely precipitation, water discharge from channels, surface runoff from surrounding sub-basins of the lagoon, evaporation and pumping. The performance tests applied to the model during the calibration and validation showed a very good performance. Additionally, two simulation scenarios were proposed, namely potential pumping and adjusted pumping, which reflected different trajectories of the water balance.
The master’s thesis concluded that precipitation and evaporation were the most determinant inflow and outflow components in the water balance of La Picasa lagoon respectively. However, the flooding event in 2016/2017 was caused by a simultaneous reduction of net evaporation and an increase in water discharge, surface runoff and intermittent pumping. The simulation scenarios suggested that an optimal operation of the pumping stations could have been effective to increase the storage capacity of the lagoon. However, during longer humid periods, it might not be enough to outweigh additional inflows.
REST became the go to approach when it comes to large scale distributed systems on, or outside the World Wide Web. This paper aims to give a brief overview of what REST is and what its main draws and benefits are. Secondly, I will showcase the implementation of REST using HTTP and why this approach became as popular as it is today. Based on my research I concluded that REST’s advantages in scalability, coupling, performance and its seamless integration with HTTP enabled it to rightfully overtake classic RPC based approaches.
Risk-based authentication (RBA) is an adaptive security measure to strengthen password-based authentication. RBA monitors additional implicit features during password entry such as device or geolocation information, and requests additional authentication factors if a certain risk level is detected. RBA is recommended by the NIST digital identity guidelines, is used by several large online services, and offers protection against security risks such as password database leaks, credential stuffing, insecure passwords and large-scale guessing attacks. Despite its relevance, the procedures used by RBA-instrumented online services are currently not disclosed. Consequently, there is little scientific research about RBA, slowing down progress and deeper understanding, making it harder for end users to understand the security provided by the services they use and trust, and hindering the widespread adoption of RBA.
In this paper, with a series of studies on eight popular online services, we (i) analyze which features and combinations/classifiers are used and are useful in practical instances, (ii) develop a framework and a methodology to measure RBA in the wild, and (iii) survey and discuss the differences in the user interface for RBA. Following this, our work provides a first deeper understanding of practical RBA deployments and helps fostering further research in this direction.
This project is focused on the generation of hardware independent code for PLCs and the comparison for energy consumption patterns of hydraulic and electric drive unit. This works is dedicated to MLC (mould level control) in a continuous casting machine, which is used to cast steel slabs continuously. The code generation is done with the help of the PLC coder which is present in the software Simulink. The programming is done entirely in MATLAB. The application of the generated code is tested on the Siemens S7-1500 PLC. For executing the code and the development of the HMI (human machine
interface) Siemens software TIA Portal V15 has been used. Moreover, for further analysis of signals and testing the code, a PDA or process data acquisition system, IBA system is used. For energy analysis also the IBA system is used.
This paper summarizes the findings on marketing automation and suitable tools, especially for companies in the Start-Up and early development phases.
It is based on Nadine Männel's bachelor thesis, which was supervised by Prof. Dr. Monika Engelen from October to December 2018. In addition to researching the basic theory of Start-Ups and Marketing Automation, 12 tools were analysed for their usability in Start-Ups.
The core content of this paper is both to create an understanding of marketing automa-tion and to derive concrete recommendations for applicable tools for marketing automation.