Novel suspension retroviral packaging cells generated by transposition using transposase encoding mRNA advance vector yields and enable production in bioreactors
- To date, the establishment of high-titer stable viral packaging cells (VPCs) at large scale for gene therapeutic applications is very time- and cost-intensive. Here we report the establishment of three human suspension 293-F-derived ecotropic MLV-based VPCs. The classic stable transfection of an EGFP-expressing transfer vector resulted in a polyclonal VPC pool that facilitated cultivation in shake flasks of 100 mL volumes and yielded high functional titers of more than 1 × 106 transducing units/mL (TU/mL). When the transfer vector was flanked by transposon terminal inverted repeats (TIRs) and upon co-transfection of a plasmid encoding for the transposase, productivities could be slightly elevated to more than 3 × 106 TU/mL. In contrast and using mRNA encoding for the transposase, as a proof of concept, productivities were drastically improved by more than ten-fold exceeding 5 × 107 TU/mL. In addition, these VPC pools were generated within only 3 weeks. The production volume was successfully scaled up to 500 mL employing a stirred-tank bioreactor (STR). We anticipate that the stable transposition of transfer vectors employing transposase transcripts will be of utility for the future establishment of high-yield VPCs producing pseudotype vector particles with a broader host tropism on a large scale.
Author: | Yasemin van Heuvel, Stefanie Schatz, Marc Hein, Tanya Dogra, Daniel Kazenmaier, Natalie Tschorn, Yvonne Genzel, Jörn Stitz |
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URN: | urn:nbn:de:hbz:832-epub4-21473 |
DOI: | https://doi.org/10.3389/fbioe.2023.1076524 |
ISSN: | 2296-4185 |
Parent Title (English): | Frontiers in Bioengineering and Biotechnology |
Publisher: | Frontiers Media S.A. |
Document Type: | Article |
Language: | English |
Date of first Publication: | 2023/04/04 |
Date of Publication (online): | 2023/05/02 |
Tag: | Gene Therapy; Murine Leukemia Virus (MLV); Retroviral Vector; Sleeping Beauty Transposon; Stirred-Tank Bioreactor; Suspension Cell; mRNA Transfection |
Volume: | 11 |
Page Number: | 10 |
Institutes: | Angewandte Naturwissenschaften (F11) |
Dewey Decimal Classification: | 500 Naturwissenschaften und Mathematik |
Open Access: | Open Access |
DeepGreen: | DeepGreen |
Licence (German): | ![]() |