Part of a Book
Refine
Document Type
- Part of a Book (18) (remove)
Language
- English (18) (remove)
Has Fulltext
- yes (18)
Keywords
- Biogas (5)
- :metabolon (2)
- CALB (2)
- Esterification (2)
- Industrial biogas plant (2)
- Lipase (2)
- Nitrification (2)
- On-demand production (2)
- Acceptor reaction (1)
- Algorithm (1)
Faculty
- Angewandte Naturwissenschaften (F11) (9)
- Fakultät 10 / :metabolon Institut (6)
- Fakultät 09 / Cologne Institute for Renewable Energy (2)
- Fakultät 09 / Institut für Bau- und Landmaschinentechnik (1)
- Fakultät 10 / Institut für Automation & Industrial IT (1)
- Fakultät 12 / Institut für Technologie und Ressourcenmanagement in den Tropen und Subtropen (1)
The synthesis of 17-hydroxy-oleic acid based oligomeric esters was investigated with immobilized Pseudozyma antarctica Lipase B and hexanediol as co-substrate. The effects of different reaction parameters on velocity and product composition at equilibrium conditions were analyzed. The synthesis of oleic acid esters was used as a reference system for initial evaluation of reaction parameters. The reaction with oleic acid and hexanediol was fastest at an enzyme concentration of 5% at 60 °C and high conversions of > 90 % were achieved in non-polar solvents in the presence of molecular sieves. In heptane an oleic acid conversion of 96 % was reached with a final diester to monoester ratio of > 4:1. In syntheses trials with 17-hydroxy-oleic acid the formation of oligomers was verified with GPC, however; conversion was generally lower than with oleic acid. Removal of hydroxyl fatty acid monomers and dimers and the formation ester functionalities could be verified by GC analysis. An increase of the degree of oligomerization was observed simultaneously by GPC analysis. The number-average molecular weight was around 1400 in the best trials corresponding to a degree of oligomerization of around 4 units of hydroxyl-fatty acid attached to a hexanediol core. Though transformations were not complete, the final oligomer size was in the lower range of polyester diols used for polyurethane manufacturing.
Online-measurement systems for agricultural and industrial AD plants – A review and practice test
(2014)
Online-measurement systems for AD plants in general are crucial to allow for detailed and comprehensive process monitoring and provide a basis for the development and practical application of process optimisation and control strategies.
Nevertheless, the online measurement of key process variables such as Volatile Fatty Acids (VFA) and Total Alkalinity (TA) has proven to be difficult due to extreme process conditions. High Total Solids (TS) concentrations and extraneous material often damage the sensors or have a strong negative impact on measurement quality and long-term behaviour.
Consequently, there is a need for new robust and accurate online-measurement systems.
The purpose of this paper is to give an overview of existing online-measurement systems, to present the current state of research and to show the results of practice tests at an agricultural and industrial AD plant. It becomes obvious that a broad variety of measurement solutions have been developed over the past few years, but that the main problem is the upscaling from lab-scale to practical application at full-scale AD plants. Results from the practice tests show that an online-measurement of pH, ORP, TS is possible.
Aerobic microbial cultivations are industrially important group of processes and pose challenges for the reactor design. In particular, estimation of industrial scale conditions is difficult from laboratory and pilot scale data. Due to complex interaction of gas/liquid phase hydrodynamics, mass transfer parameters and microbial metabolism, both improvement of modelling tools and reactor design are desired. We present an approach to estimate growth conditions in industrial scale reactor by combining black-box metabolic models with CFD-model.
The reactor type used here is Outotec OKTOP9000®, which is used in the industrial hydrometallurgical processes at 900 m3 scale. It is adopted to a laboratory setting and compared to stirred tank reactor (STR) in gas dispersion, mass transfer and yeast cultivation experiments. In addition, a kinetic model for the yeast growth is developed based on literature sources and validated by the laboratory scale batch cultivations. This kinetic model is used along with CFD-model that is developed to describe the flow and mass transfer conditions in the industrial scale reactor.
The laboratory scale experiments show the feasibility of OKTOP9000® reactor when compared to STR, particularly with improved gas handling capacity. The modelling approach shows qualitatively similar behavior in the large scale simulations when compared to laboratory scale cultivations.
In the degradation of ammonia (NH4+) to gaseous nitrogen (N2), the nitrification is one of the two reaction steps. The nitrification itself is divided in two steps and is performed by two different types of bacteria. Current literature has shown that there are types of bacteria, which have the genetic equipment to perform both steps in one bacteria. Nevertheless, in wastewater and landfill leachate treatment, ammonia-oxidizing organisms (AOO) and nitrite-oxidizing organisms (NOO) occur as a symbiosis. The intermediate of the two consecutive reaction steps (NO2-, nitrite) is toxic. For this reason, both steps are necessary for the two bacterial groups. To determine the ratio of AOO, NOO and heterotrophic bacteria (which use organic compounds as carbon and energy source) the oxygen uptake rate (OUR) with selective inhibition with N-allylthiourea (ATU) and azide is used. In the inflow of a pilot plant in one street a step by step increased amount of a process water out of a fermentation plant was added to the landfill leachate. For comparison, the other street was supplied only with landfill leachate with the same amount of nitrogen. As a result, comparable values for the different bacterial groups and reproducible results were measured and lead to a better understanding of the analysed nitrification sludge. Deeper understanding of the behavior of the different groups will result in a reduce risk of malfunctions and a more stable operation in the wastewater or landfill leachate treatment plant.
Due to the worldwide shortage of petrochemical based resources, the usage of renewable bio-based raw materials for established and novel products becomes increasingly important.[1] Such bio-based resources are already used for the fabrication of a variety of products, e. g. paper, lubricants, detergents or cosmetics. In the future they are expected to emerge in many more applications in industry and household.[1]
A very promising approach relies on the use of glycolipids as a source of hydroxy-oleic acid.[2] Microbial glycolipids are produced for instance via fermentation from natural resources such as plant oils and sugar.[3] After fermentation complex product mixtures are obtained with the composition depending on the microorganism, substrate and fermentation time.[3] The successful use of microbial glycolipids and hydroxy-oleic acid (HOA) derived therefrom as bio-based intermediates requires reliable analytical methods as well as robust manufacturing processes for the synthesis and cleavage of bio-based molecules. In order to obtain hydroxy-oleic acids as bio-based intermediates, the acidic cleavage of microbial derived sophorolipid was investigated. In addition the implementation of HOA in polyurethane (PU) systems was explored.
For use in a landfill, a laboratory reactor for safe and environmentally friendly biological utilization of low-concentration methane gas will be further developed. The current principle of denitrification-coupled aerobic methane oxidation will be replaced by methane oxidation under anaerobic conditions. Anaerobic methane oxidation offers the advantage that, in addition to methane, nitrate also undergoes biodegradation. Another advantage is that the oxygen content can be significantly lower. This reduces the risk of the formation of an explosive atmosphere in the reactor. Currently, the principle of anaerobic methane oxidation is known. However, organisms capable of doing so are not yet available as a pure culture. Therefore, several biomasses were probed for the ability of anaerobic methane oxidation. It was found that moor-heavy sediment, activated sludge from the leachate treatment plant and biomass from the local biogas plant oxidize methane after the natural carbon source (C source) was been removed.
This paper presents a series of flow and temperature measurements on the principal heat network of :metabolon in Lindlar, Germany. These measurements intend to show the behaviour of the system on specific production areas of :metabolon for future monitoring and optimisation purposes. Such measurements allow the analysis of the system’s heat flow through the network, which showed that losses exist, some areas. The results demonstrate successfully that the temperature and flow changes deserve more detailed and fixed monitoring in specific areas to help the user decide the optimum measuring point.
Comparative analysis of non-natural acceptor glucosylation with sucrase enzymes of family GH 70
(2019)
Mutan- and alternansucrase were analyzed for their non-natural glucosylation potential with catecholic compounds caffeic acid and nordihydroguaiaretic acid (NDGA) as well as with non-catecolic p-coumaric acid and umbellic acid. Mutansucrase accepted both catecholic substrates and high glucosylation yields of 92 % with caffeic acid and 81 % with NDGA were obtained. The enzyme showed a clear regio-preference for the catechol 4-OH, which corresponds to findings from our previous work with Leuconostoc and Weissella derived glucansucrases. The substrate spectrum of the alternansucrase was broader and all substrates were successfully glucosylated with a preference for the catechols. Interestingly alternansucrase possessed a different regio-specificity. With caffeic acid the 3-O-α-D-glucoside was the major product. A similar substrate spectrum and regioselectivity pattern was observed in previous glucansucrase screenings only with glucansucrase from strain Weissella beninensis DSM 22752. Therefore it may be concluded that the W. beninensis enzyme is an alternansucrase type enzyme as well.
The utilization of roadside-green-cuttings (grass) for anaerobic digestion increases provides an additional possible source of organic waste for use as a renewable energy source. Grass can be used as a substrate to increase biogas yield. Nevertheless, the anaerobic digestion of this kind of waste can be limited due to the fact that it could be contaminated with heavy metals, in particular from traffic emissions and industrial activity. For this reason the biogas production of grass from a busy road was assessed. Samples of roadside-grass were washed with an organosulphide, which is used for the removal of heavy metals from wastewater.
A comparison of the anaerobic digestion of washed and unwashed roadside grass was performed. Results showed that the anaerobic digestion of the unwashed grass was much more effective than the washed grass. A second experiment was carried out and co-fermentation of manure and farm-grass was prepared for anaerobic digestion. Lead was added in the concentrations 500, 1000 and 2000 mg Pb2+/kg. The results showed that the higher the lead concentration, the lower the inhibition of the biogas yield. The grass could be acting as phytoremediator for high lead concentrations. The grass could contain organic compounds, which can as-similate heavy metals.
In the last few decades raw material molasses, used in large scale fermentations in the production of bioethanol, citric acid, (baker´s) yeast and yeast extracts, has become more and more expensive. That is why agro-industrial wastes have become an interesting alternative. They are being produced in large volumes every day and represent a serious environmental problem considering its high organic content. The present contribution aims to demonstrate how waste products of wine production can be employed as substrate in bioethanol production. Cultivation of yeast and bioethanol production on molasses and grape pomace extract was studied in flasks in laboratory scale. This work should be regarded as an example of integrated sustainability which demonstrates how the waste from one industrial process is used as feedstock for another.